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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using KMO Recombinant antibody (83812-1-RR)

    WB analysis of MCF-7 using 83812-1-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83812-1-RR (KMO antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using KMO Recombinant antibody (83812-1-RR)

    IHC staining of mouse kidney using 83812-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using KMO Recombinant antibody (83812-1-RR)

    IHC staining of mouse kidney using 83812-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KMO Recombinant antibody (83812-1-RR)

    IHC staining of mouse brain using 83812-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using KMO Recombinant antibody (83812-1-RR)

    IHC staining of mouse heart using 83812-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of MCF-7 cells using KMO Recombinant antibody (83812-1-RR)

    FC experiment of MCF-7 using 83812-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug KMO Recombinant antibody (83812-1-RR, Clone:240586D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83812-1-RR

    Affinity and Kinetic Characterization of 83812-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83812-1-RR against Human KMO were performed. The affinity constant is below 1 pM.

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KMO Recombinant antibody

KMO Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83812-1-RR
Clone No.240586D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

Kynurenine 3-monooxygenase, Kynurenine 3-hydroxylase, Kynurenine 3 monooxygenase, Kynurenine 3 hydroxylase, EC:1.14.13.9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inmouse kidney tissue, mouse brain tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83812-1-RR targets KMO in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KMO fusion protein Ag35029 相同性解析による交差性が予測される生物種
Full Name kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
Calculated molecular weight486 aa, 56 kDa
Observed molecular weight52-56 kDa
GenBank accession numberBC005297
Gene Symbol KMO
Gene ID (NCBI) 8564
RRIDAB_3671400
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15229
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KMO(Kynurenine 3-monooxygenase) is an NADPH-dependent flavin monooxygenase, catalysing the hydroxylation of the L-kynurenine to form L-3-hydroxykynurenine. KMO is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).

Protocols

Product Specific Protocols
WB protocol for KMO antibody 83812-1-RRDownload protocol
IHC protocol for KMO antibody 83812-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols