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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KPNA2 Monoclonal antibody, PBS Only

KPNA2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G9C1

Cat no : 66870-1-PBS

Synonyms

Karyopherin subunit alpha-2, IPOA1, IPOA 1, IPO A1, Importin subunit alpha-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KPNA2 Monoclonal antibody (66870-1-Ig)

    WB analysis using 66870-1-Ig (same clone as 66870-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66870-1-Ig (KPNA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66870-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using KPNA2 Monoclonal antibody (66870-1-Ig)

    IHC staining of human skin cancer using 66870-1-Ig (same clone as 66870-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66870-1-Ig (KPNA2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66870-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using KPNA2 Monoclonal antibody (66870-1-Ig)

    IF Staining of MCF-7 using 66870-1-Ig (same clone as 66870-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KPNA2 antibody (66870-1-Ig, Clone: 2G9C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66870-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66870-1-PBS targets KPNA2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KPNA2 fusion protein Ag28386 相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
Calculated molecular weight 57.8 kDa
Observed molecular weight 55-58 kDa
GenBank accession numberBC005978
Gene symbol KPNA2
Gene ID (NCBI) 3838
RRIDAB_2882207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

KPNA2 (karyopherin α2) is a nuclear import factor involved in the nucleocytoplasmic transport system. It is functionally involved in the tumor progression and is elevated in multiple cancers. KPNA2 expression may be a useful prognostic biomarker to monitor cancer prognosis.