• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using KPNA2 Monoclonal antibody (66870-1-Ig)

    WB analysis using 66870-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66870-1-Ig (KPNA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using KPNA2 Monoclonal antibody (66870-1-Ig)

    IHC staining of human skin cancer using 66870-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66870-1-Ig (KPNA2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using KPNA2 Monoclonal antibody (66870-1-Ig)

    IF Staining of MCF-7 using 66870-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KPNA2 antibody (66870-1-Ig, Clone: 2G9C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

KPNA2 Monoclonal antibody

KPNA2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66870-1-Ig
Clone No.2G9C1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Karyopherin subunit alpha-2, IPOA1, IPOA 1, IPO A1, Importin subunit alpha-1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, Raji cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66870-1-Ig targets KPNA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KPNA2 fusion protein Ag28386 相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
Calculated molecular weight 57.8 kDa
Observed molecular weight 55-58 kDa
GenBank accession numberBC005978
Gene Symbol KPNA2
Gene ID (NCBI) 3838
RRIDAB_2882207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52292
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KPNA2 (karyopherin α2) is a nuclear import factor involved in the nucleocytoplasmic transport system. It is functionally involved in the tumor progression and is elevated in multiple cancers. KPNA2 expression may be a useful prognostic biomarker to monitor cancer prognosis.

Protocols

Product Specific Protocols
WB protocol for KPNA2 antibody 66870-1-IgDownload protocol
IHC protocol for KPNA2 antibody 66870-1-IgDownload protocol
IF protocol for KPNA2 antibody 66870-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols