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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated KPNA2 Monoclonal antibody

KPNA2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2G9C1

Cat no : CL488-66870

Synonyms

Karyopherin subunit alpha-2, IPOA1, IPOA 1, IPO A1, Importin subunit alpha-1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated KPNA2 Monoclonal ant (CL488-66870)

    IF Staining of MCF-7 using CL488-66870

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 KPNA2 antibody (CL488-66870, Clone: 2G9C1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HEK-293T cells using CoraLite® Plus 488-conjugated KPNA2 Monoclonal ant (CL488-66870)

    FC experiment of HEK-293T using CL488-66870

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human KPNA2 (CL488-66870, Clone:2G9C1) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66870 targets KPNA2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KPNA2 fusion protein Ag28386 相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
Calculated molecular weight 57.8 kDa
Observed molecular weight 55-58 kDa
GenBank accession numberBC005978
Gene symbol KPNA2
Gene ID (NCBI) 3838
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KPNA2 (karyopherin α2) is a nuclear import factor involved in the nucleocytoplasmic transport system. It is functionally involved in the tumor progression and is elevated in multiple cancers. KPNA2 expression may be a useful prognostic biomarker to monitor cancer prognosis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 KPNA2 antibody CL488-66870Download protocol
FC protocol for CL Plus 488 KPNA2 antibody CL488-66870Download protocol
Standard Protocols
Click here to view our Standard Protocols