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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KPNA3 Monoclonal antibody (67892-1-Ig)

    WB analysis using 67892-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67892-1-Ig (KPNA3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HepG2 cells using KPNA3 Monoclonal antibody (67892-1-Ig)

    WB analysis of HepG2 using 67892-1-Ig

    WB result of KPNA3 antibody (67892-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KPNA3 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using KPNA3 Monoclonal antibody (67892-1-Ig)

    WB analysis using 67892-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67892-1-Ig (KPNA3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KPNA3 Monoclonal antibody (67892-1-Ig)

    IHC staining of mouse testis using 67892-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67892-1-Ig (KPNA3 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KPNA3 Monoclonal antibody (67892-1-Ig)

    IHC staining of mouse testis using 67892-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67892-1-Ig (KPNA3 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • KD/KO Validated

KPNA3 Monoclonal antibody

KPNA3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67892-1-Ig
Clone No.1D3D2
Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

hSRP1, Importin alpha Q2, Importin subunit alpha 3, IPOA4, Karyopherin subunit alpha 3, KPNA3, Qip2, SRP1, SRP1 gamma, SRP1gamma, SRP4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67892-1-Ig targets KPNA3 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen KPNA3 fusion protein Ag27767 相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 3 (importin alpha 4)
Calculated molecular weight 58 kDa
Observed molecular weight58 kDa
GenBank accession numberBC017355
Gene Symbol KPNA3
Gene ID (NCBI) 3839
RRIDAB_2918648
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00505
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

karyopherin subunit alpha 3 (KPNA3) is a member of the importin alpha family which can transport molecules between the mucleus and cytoplasm. It is ubiquitous expressed in brain,testis and esophagus. KPNA3 is associated with many severe diseases such as hepatocellular Carcinoma (PMID: 31417635) , schizophrenia, major depression and opiate dependence (PMID: 22960338) . The molecular weight of KPNA3 is about 58 kDa.

Protocols

Product Specific Protocols
WB protocol for KPNA3 antibody 67892-1-IgDownload protocol
IHC protocol for KPNA3 antibody 67892-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

MERS-CoV-nsp5 expression in human epithelial BEAS 2b cells attenuates type I interferon production by inhibiting IRF3 nuclear translocation

Authors - Y Zhang
View Article