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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KPNA3 Monoclonal antibody (67892-1-Ig)

    WB analysis using 67892-1-Ig (same clone as 67892-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67892-1-Ig (KPNA3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67892-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using KPNA3 Monoclonal antibody (67892-1-Ig)

    WB analysis of HepG2 using 67892-1-Ig (same clone as 67892-1-PBS)

    WB result of KPNA3 antibody (67892-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KPNA3 transfected HepG2 cells. This data was developed using the same antibody clone with 67892-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KPNA3 Monoclonal antibody (67892-1-Ig)

    WB analysis using 67892-1-Ig (same clone as 67892-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67892-1-Ig (KPNA3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67892-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KPNA3 Monoclonal antibody (67892-1-Ig)

    IHC staining of mouse testis using 67892-1-Ig (same clone as 67892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67892-1-Ig (KPNA3 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67892-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KPNA3 Monoclonal antibody (67892-1-Ig)

    IHC staining of mouse testis using 67892-1-Ig (same clone as 67892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67892-1-Ig (KPNA3 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67892-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

KPNA3 Monoclonal antibody, PBS Only

KPNA3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67892-1-PBS
Clone No.1D3D2

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

WB, IHC, ELISA

Synonyms

hSRP1, Importin alpha Q2, Importin subunit alpha 3, IPOA4, Karyopherin subunit alpha 3, KPNA3, Qip2, SRP1, SRP1 gamma, SRP1gamma, SRP4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67892-1-PBS targets KPNA3 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen KPNA3 fusion protein Ag27767 相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 3 (importin alpha 4)
Calculated molecular weight 58 kDa
Observed molecular weight58 kDa
GenBank accession numberBC017355
Gene Symbol KPNA3
Gene ID (NCBI) 3839
RRIDAB_2918648
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00505
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

karyopherin subunit alpha 3 (KPNA3) is a member of the importin alpha family which can transport molecules between the mucleus and cytoplasm. It is ubiquitous expressed in brain,testis and esophagus. KPNA3 is associated with many severe diseases such as hepatocellular Carcinoma (PMID: 31417635) , schizophrenia, major depression and opiate dependence (PMID: 22960338) . The molecular weight of KPNA3 is about 58 kDa.