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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KPNA4 Recombinant antibody (82898-1-RR)

    WB analysis using 82898-1-RR (same clone as 82898-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82898-1-RR (KPNA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82898-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using KPNA4 Recombinant antibody (82898-1-RR)

    WB analysis of A549 using 82898-1-RR (same clone as 82898-1-PBS)

    WB result of KPNA4 antibody (82898-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KPNA4 transfected A549 cells. This data was developed using the same antibody clone with 82898-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using KPNA4 Recombinant antibody (82898-1-RR)

    IF Staining of U2OS using 82898-1-RR (same clone as 82898-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KPNA4 antibody (82898-1-RR, Clone: 230098H3 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82898-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using KPNA4 Recombinant antibody (82898-1-RR)

    FC experiment of HeLa using 82898-1-RR (same clone as 82898-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.13 ug Anti-Human KPNA4 (82898-1-RR, Clone:230098H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.13 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82898-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82898-1-RR

    Affinity and Kinetic Characterization of 82898-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82898-1-RR against Human KPNA4 were performed. The affinity constant is 0.417 nM.

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  • Featured Product
  • KD/KO Validated

KPNA4 Recombinant antibody, PBS Only

KPNA4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82898-1-PBS
Clone No.230098H3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

QIP1, KPNA 4, Karyopherin subunit alpha-4, IPOA3, Importin subunit alpha-3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82898-1-PBS targets KPNA4 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KPNA4 fusion protein Ag3133 相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 4 (importin alpha 3)
Calculated molecular weight 521 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC034493
Gene Symbol KPNA4
Gene ID (NCBI) 3840
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00629
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.