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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KPNA4 Recombinant antibody, PBS Only

KPNA4 Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230098H3

Cat no : 82898-1-PBS

Synonyms

QIP1, KPNA 4, Karyopherin subunit alpha-4, IPOA3, Importin subunit alpha-3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using KPNA4 Recombinant antibody (82898-1-RR)

    IF Staining of U2OS using 82898-1-RR (same clone as 82898-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KPNA4 antibody (82898-1-RR, Clone: 230098H3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82898-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using KPNA4 Recombinant antibody (82898-1-RR)

    FC experiment of HeLa using 82898-1-RR (same clone as 82898-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.13 ug Anti-Human KPNA4 (82898-1-RR, Clone:230098H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.13 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82898-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82898-1-RR

    Affinity and Kinetic Characterization of 82898-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82898-1-RR against Human KPNA4 were performed. The affinity constant is 0.417 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82898-1-PBS targets KPNA4 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KPNA4 fusion protein Ag3133 相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 4 (importin alpha 3)
Calculated molecular weight 521 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC034493
Gene symbol KPNA4
Gene ID (NCBI) 3840
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.