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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KPNA4 Recombinant antibody (82898-1-RR)

    WB analysis using 82898-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82898-1-RR (KPNA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using KPNA4 Recombinant antibody (82898-1-RR)

    WB analysis of A549 using 82898-1-RR

    WB result of KPNA4 antibody (82898-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KPNA4 transfected A549 cells.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using KPNA4 Recombinant antibody (82898-1-RR)

    IF Staining of U2OS using 82898-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KPNA4 antibody (82898-1-RR, Clone: 230098H3 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using KPNA4 Recombinant antibody (82898-1-RR)

    FC experiment of HeLa using 82898-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.13 ug Anti-Human KPNA4 (82898-1-RR, Clone:230098H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.13 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82898-1-RR

    Affinity and Kinetic Characterization of 82898-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82898-1-RR against Human KPNA4 were performed. The affinity constant is 0.417 nM.

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  • Featured Product
  • KD/KO Validated

KPNA4 Recombinant antibody

KPNA4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 82898-1-RR
Clone No.230098H3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

QIP1, KPNA 4, Karyopherin subunit alpha-4, IPOA3, Importin subunit alpha-3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A549 cells, Jurkat cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82898-1-RR targets KPNA4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KPNA4 fusion protein Ag3133 相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 4 (importin alpha 3)
Calculated molecular weight 521 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC034493
Gene Symbol KPNA4
Gene ID (NCBI) 3840
RRIDAB_3670626
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00629
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KPNA4 antibody 82898-1-RRDownload protocol
IF protocol for KPNA4 antibody 82898-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols