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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated KPNA4 Recombinant antibody

KPNA4 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230098H1

Cat no : CL488-82898-2

Synonyms

Importin alpha Q1, Importin subunit alpha 4, IPOA3, Karyopherin subunit alpha 4, KPNA4, QIP1, SRP3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated KPNA4 Recombinant an (CL488-82898-2)

    IF Staining of U2OS using CL488-82898-2

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 KPNA4 antibody (CL488-82898-2, Clone: 230098H1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of U2OS cells using CoraLite® Plus 488-conjugated KPNA4 Recombinant an (CL488-82898-2)

    FC experiment of U2OS using CL488-82898-2

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated KPNA4 Recombinant antibody (CL488-82898-2, Clone:230098H1)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82898-2 targets KPNA4 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KPNA4 fusion protein Ag3133 相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 4 (importin alpha 3)
Calculated molecular weight 521 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC034493
Gene symbol KPNA4
Gene ID (NCBI) 3840
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KPNA4, also named importin subunit alpha-3, karyopherin-alpha4, is a cytoplasmic protein that recognizes nuclear localization signals (NLSs) and dock NLS-containing proteins to the nuclear pore complex. Nuclear import, mediated in part by karyopherin-α (KPNA)/importin-α subtypes, regulates transcription factor access to the genome and determines cell fate. KPNA4-mediated nuclear transport of Ras-responsive element-binding protein (RREB1), which sustains Ras/ERK pathway signaling through repressing miR-143/145 expression (PMID: 31822798). KPNA4 is one of the main isoforms that is activated in many human cancers. KPNA4 expression was elevated in head and neck of squamous cell carcinoma (PMID: 33188837).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 KPNA4 antibody CL488-82898-2Download protocol
Standard Protocols
Click here to view our Standard Protocols