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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KRAS-2A-specific Monoclonal antibody (67266-1-Ig)

    WB analysis using 67266-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KRAS-2A-specific Monoclonal antibody (67266-1-Ig)

    WB analysis using 67266-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KRAS-4A-specific Monoclonal antibody (67266-1-Ig)

    IF Staining of HeLa using 67266-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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KRAS-4A-specific Monoclonal antibody

KRAS-4A-specific Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67266-1-Ig
Clone No.3C8F5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

KRAS-4A, KRAS, pan RAS, EC:3.6.5.2, c-K-ras

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, SH-SY5Y cells, PC-12 cells, Neuro-2a cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67266-1-Ig targets KRAS-4A-specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight 21 kDa
Observed molecular weight 22 kDa
GenBank accession numberNM_033360
Gene Symbol KRAS
Gene ID (NCBI) 3845
RRIDAB_2882537
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01116
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAS (derived from 'RAt Sarcoma' virus) gene family includes KRAS, HRAS, and NRAS. These genes encode very similar 21 kDa proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 4A and 4B. This antibody is specific for KRAS-4A, which is also named as KRAS-2A.

Protocols

Product Specific Protocols
WB protocol for KRAS-4A-specific antibody 67266-1-IgDownload protocol
IF protocol for KRAS-4A-specific antibody 67266-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols