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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KRAS-2A-specific Monoclonal antibody, PBS Only

KRAS-2A-specific Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C8F5

Cat no : 67266-1-PBS

Synonyms

C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2A, KRAS-2A-specific, KRAS2B, KRAS2B speicifc, Krasa, Krasb, NS3, RASK2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KRAS-2A-specific Monoclonal antibody (67266-1-Ig)

    WB analysis using 67266-1-Ig (same clone as 67266-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67266-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KRAS-2A-specific Monoclonal antibody (67266-1-Ig)

    WB analysis using 67266-1-Ig (same clone as 67266-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67266-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KRAS-2A-specific Monoclonal antibody (67266-1-Ig)

    IF Staining of HeLa using 67266-1-Ig (same clone as 67266-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67266-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67266-1-PBS targets KRAS-2A-specific in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight 21 kDa
Observed molecular weight 22 kDa
GenBank accession numberNM_033360
Gene symbol KRAS
Gene ID (NCBI) 3845
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.