Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 12063-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
WB analysis using 12063-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12063-1-AP
WB result of KRAS antibody (12063-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KRAS transfected HEK-293 cells.
WB analysis using 12063-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.
WB analysis using 12063-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12063-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 12063-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 12063-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12063-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 12063-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12063-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of MKN-45 using 12063-1-AP
IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:8000) with MKN-45 cells lysate 1560 ug.
IP experiment of HGC-27 using 12063-1-AP
IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:8000) with HGC-27 cells lysate 1520 ug.
IP experiment of mouse brain using 12063-1-AP
IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:20000) with mouse brain tissue lysate 1720 ug.
IP experiment of HeLa using 12063-1-AP
IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:20000) with HeLa cells lysate 1200 ug.
IHC staining of human lung cancer using 12063-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12063-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 12063-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 12063-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 12063-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 12063-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 12063-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 12063-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KRAS antibody (12063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 12063-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KRAS antibody (12063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 12063-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRAS antibody (12063-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 12063-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KRAS (12063-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

KRAS Polyclonal antibody

KRAS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 12063-1-AP

Publications(158)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

pan RAS, C K RAS, c Ki ras, c-Ki-ras, c-K-ras

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (158)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, HEK-293 cells, human brain tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, rat liver tissue, mouse brain tissue, rat brain tissue
Positive IP detected in	HeLa cells, HGC-27 cells, MKN-45 cells, mouse brain tissue
Positive IHC detected in	human lung cancer tissue, human kidney tissue, human pancreas cancer tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 15 publications below
WB	See 140 publications below
IHC	See 23 publications below
IF	See 13 publications below
IP	See 2 publications below

Product Information

12063-1-AP targets KRAS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, chicken, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2700 Product name: Recombinant human KRAS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-188 aa of BC013572 Sequence: MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM 相同性解析による交差性が予測される生物種
Full Name	v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight	188 aa, 21 kDa
Observed molecular weight	21 kDa
GenBank accession number	BC013572
Gene Symbol	KRAS
Gene ID (NCBI)	3845
RRID	AB_878040
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P01116
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.

Protocols

Product Specific Protocols
IF protocol for KRAS antibody 12063-1-AP	Download protocol
IHC protocol for KRAS antibody 12063-1-AP	Download protocol
IP protocol for KRAS antibody 12063-1-AP	Download protocol
WB protocol for KRAS antibody 12063-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Biotechnol Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors. Authors - Christopher W Davies View Article
human	WB	Cancer Discov LZTR1 mutation mediates oncogenesis through stabilization of EGFR and AXL Authors - Aram Ko View Article
mouse	WB,IHC	Cell Res In vivo self-assembled small RNAs as a new generation of RNAi therapeutics. Authors - Zheng Fu View Article
mouse	WB	Cancer Discov Systematic functional interrogation of rare cancer variants identifies oncogenic alleles. Authors - Eejung Kim View Article
human	WB	Mol Cell The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation. Authors - Linyu Sun View Article
human	WB	Mol Cell Regulation of GTPase function by autophosphorylation. Authors - Christian W Johnson View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12063-1-AP

WB analysis using 12063-1-AP

WB analysis of HEK-293 using 12063-1-AP

WB analysis using 12063-1-AP

WB analysis using 12063-1-AP

WB analysis of human kidney using 12063-1-AP

WB analysis of rat kidney using 12063-1-AP

WB analysis of rat liver using 12063-1-AP

WB analysis of human brain using 12063-1-AP

WB analysis of mouse kidney using 12063-1-AP

WB analysis of A549 using 12063-1-AP

IP experiment of MKN-45 using 12063-1-AP

IP experiment of HGC-27 using 12063-1-AP

IP experiment of mouse brain using 12063-1-AP

IP experiment of HeLa using 12063-1-AP

IHC staining of human lung cancer using 12063-1-AP

IHC staining of human lung cancer using 12063-1-AP

IHC staining of human kidney using 12063-1-AP

IHC staining of human kidney using 12063-1-AP

IHC staining of mouse kidney using 12063-1-AP

IHC staining of human pancreas cancer using 12063-1-AP

IHC staining of human pancreas cancer using 12063-1-AP

IF Staining of mouse brain using 12063-1-AP

IF Staining of mouse brain using 12063-1-AP

IF Staining of HeLa using 12063-1-AP

FC experiment of HeLa using 12063-1-AP

KRAS Polyclonal antibody

KRAS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors.

LZTR1 mutation mediates oncogenesis through stabilization of EGFR and AXL

In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.

Systematic functional interrogation of rare cancer variants identifies oncogenic alleles.

The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.

Regulation of GTPase function by autophosphorylation.