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  • Featured Product
  • KD/KO Validated

KRAS Polyclonal antibody, PBS Only

KRAS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 12063-1-PBS

Synonyms

KRAS4A, KRAS4B, pan RAS, GTPase KRas, EC:3.6.5.2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of HEK-293 using 12063-1-AP (same clone as 12063-1-PBS)

    WB result of KRAS antibody (12063-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KRAS transfected HEK-293 cells. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of A549 using 12063-1-AP (same clone as 12063-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of rat liver using 12063-1-AP (same clone as 12063-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of human brain using 12063-1-AP (same clone as 12063-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of human kidney using 12063-1-AP (same clone as 12063-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of mouse kidney using 12063-1-AP (same clone as 12063-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of rat kidney using 12063-1-AP (same clone as 12063-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using KRAS Polyclonal antibody (12063-1-AP)

    IP experiment of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:10000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human lung cancer using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human lung cancer using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human kidney using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human kidney using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human pancreas cancer using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human pancreas cancer using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KRAS Polyclonal antibody (12063-1-AP)

    IF Staining of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KRAS antibody (12063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KRAS Polyclonal antibody (12063-1-AP)

    IF Staining of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KRAS antibody (12063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KRAS Polyclonal antibody (12063-1-AP)

    IF Staining of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRAS antibody (12063-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using KRAS Polyclonal antibody (12063-1-AP)

    FC experiment of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KRAS (12063-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12063-1-PBS targets KRAS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KRAS fusion protein Ag2700 相同性解析による交差性が予測される生物種
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight 188 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC013572
Gene symbol KRAS
Gene ID (NCBI) 3845
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.