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  • Featured Product
  • KD/KO Validated

Cytokeratin 13 Polyclonal antibody

Cytokeratin 13 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10164-2-AP

Synonyms

KRT13, Cytokeratin-13, Cytokeratin,Keratin, ck17, CK-13

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Cytokeratin 13 Polyclonal antibody (10164-2-AP)

    WB analysis of A431 using 10164-2-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using Cytokeratin 13 Polyclonal antibody (10164-2-AP)

    WB analysis of A431 using 10164-2-AP

    WB result of Cytokeratin 13 antibody (10164-2-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 13 transfected A431 cells.

  • Western Blot (WB) analysis of mouse skin tissue using Cytokeratin 13 Polyclonal antibody (10164-2-AP)

    WB analysis of mouse skin using 10164-2-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 10164-2-AP (Cytokeratin 13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using Cytokeratin 13 Polyclonal antibody (10164-2-AP)

    IP experiment of A431 using 10164-2-AP

    IP result of anti-Cytokeratin 13 (IP:10164-2-AP, 4ug; Detection:10164-2-AP 1:1000) with A431 cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 13 Polyclonal antibody (10164-2-AP)

    IHC staining of human cervical cancer using 10164-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 13 Polyclonal antibody (10164-2-AP)

    IHC staining of human cervical cancer using 10164-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using Cytokeratin 13 Polyclonal antibody (10164-2-AP)

    IHC staining of human oesophagus using 10164-2-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using Cytokeratin 13 Polyclonal antibody (10164-2-AP)

    IHC staining of human oesophagus using 10164-2-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using Cytokeratin 13 Polyclonal antibody (10164-2-AP)

    IF Staining of HaCaT using 10164-2-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 13 antibody (10164-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cytokeratin 13 Polyclonal antibody (10164-2-AP)

    IF Staining of A431 using 10164-2-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 13 antibody (10164-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of A431 cells using Cytokeratin 13 Polyclonal antibody (10164-2-AP)

    FC experiment of A431 using 10164-2-AP

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 13 (10164-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA431 cells, mouse skin tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman cervical cancer tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells, A431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10164-2-AP targets Cytokeratin 13 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 13 fusion protein Ag0217 相同性解析による交差性が予測される生物種
Full Name keratin 13
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC002661
Gene symbol Cytokeratin 13
Gene ID (NCBI) 3860
RRIDAB_2134679
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 13 antibody 10164-2-APDownload protocol
IHC protocol for Cytokeratin 13 antibody 10164-2-APDownload protocol
IF protocol for Cytokeratin 13 antibody 10164-2-APDownload protocol
IP protocol for Cytokeratin 13 antibody 10164-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Theranostics

Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p

Authors - Soo Hyun Kang
View Article
humanIF

Elife

Chloride channels regulate differentiation and barrier functions of the mammalian airway.

Authors - Mu He
View Article
humanWB

Cell Commun Signal

Liprin-α1 modulates cancer cell signaling by transmembrane protein CD82 in adhesive membrane domains linked to cytoskeleton.

Authors - Henna Pehkonen
View Article
humanIF

Am J Respir Cell Mol Biol

Do airway epithelium air-liquid cultures represent the in vivo airway epithelium transcriptome?

Authors - Dvorak Anna A
View Article
humanIF

Stem Cells Int

Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.

Authors - Cong Li
View Article
humanIHC

Mol Vis

Expression analysis of human pterygium shows a predominance of conjunctival and limbal markers and genes associated with cell migration.

Authors - Jaworski C J CJ
View Article