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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cytokeratin 13 Polyclonal antibody

Cytokeratin 13 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10164

Synonyms

KRT13, Cytokeratin-13, Cytokeratin,Keratin, ck17, CK-13

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated Cytokeratin 13 Polyc (CL488-10164)

    IF Staining of A431 using CL488-10164

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite® Plus 488 Cytokeratin 13 antibody (CL488-10164) at dilution of 1:400.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated Cytokeratin 13 Polyc (CL488-10164)

    FC experiment of A431 using CL488-10164

    1X10^6 A431 cells were intracellularly stained with 1.6 ug CoraLite® Plus 488 Anti-Human Cytokeratin 13 (CL488-10164) (red), or 1.6 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 1.60 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10164 targets Cytokeratin 13 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 13 fusion protein Ag0217 相同性解析による交差性が予測される生物種
Full Name keratin 13
Calculated molecular weight 50 kDa
GenBank accession numberBC002661
Gene symbol Cytokeratin 13
Gene ID (NCBI) 3860
RRIDAB_2918957
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 13 antibody CL488-10164Download protocol
Standard Protocols
Click here to view our Standard Protocols