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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 16 Polyclonal antibody

Cytokeratin 16 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17265-1-AP

Synonyms

KRT16, Keratin16, keratin 16, Cytokeratin,Keratin, CK-16

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 16 Polyclonal antibody (17265-1-AP)

    WB analysis using 17265-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using Cytokeratin 16 Polyclonal antibody (17265-1-AP)

    WB analysis of A375 using 17265-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Cytokeratin 16 Polyclonal antibody (17265-1-AP)

    WB analysis of MCF-7 using 17265-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 16 Polyclonal antibody (17265-1-AP)

    IHC staining of human oesophagus cancer using 17265-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 16 Polyclonal antibody (17265-1-AP)

    IHC staining of human oesophagus cancer using 17265-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using Cytokeratin 16 Polyclonal antibody (17265-1-AP)

    IHC staining of human bowen disease using 17265-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using Cytokeratin 16 Polyclonal antibody (17265-1-AP)

    IHC staining of human bowen disease using 17265-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin tissue using Cytokeratin 16 Polyclonal antibody (17265-1-AP)

    IHC staining of human skin using 17265-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 17265-1-AP (Cytokeratin 16 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using Cytokeratin 16 Polyclonal antibody (17265-1-AP)

    IHC staining of human skin using 17265-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 17265-1-AP (Cytokeratin 16 antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Cytokeratin 16 Polyclonal antibody (17265-1-AP)

    IHC staining of mouse skin using 17265-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Cytokeratin 16 Polyclonal antibody (17265-1-AP)

    IHC staining of mouse skin using 17265-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 16 Polyclonal antibody (17265-1-AP)

    IHC staining of human cervical cancer using 17265-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 16 Polyclonal antibody (17265-1-AP)

    IHC staining of human cervical cancer using 17265-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cytokeratin 16 Polyclonal antibody (17265-1-AP)

    IF Staining of A431 using 17265-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Cytokeratin 16 antibody (17265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, A375 cells, MCF-7 cells
Positive IHC detected inhuman oesophagus cancer tissue, human bowen disease, human cervical cancer tissue, human skin tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17265-1-AP targets Cytokeratin 16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 16 fusion protein Ag10999 相同性解析による交差性が予測される生物種
Full Name keratin 16
Calculated molecular weight 473 aa, 51 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC039169
Gene symbol Cytokeratin 16
Gene ID (NCBI) 3868
RRIDAB_2132894
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 16 antibody 17265-1-APDownload protocol
IHC protocol for Cytokeratin 16 antibody 17265-1-APDownload protocol
IF protocol for Cytokeratin 16 antibody 17265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Phytother Res

Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization

Authors - Yongling Ning
View Article
humanWB

Int J Clin Exp Pathol

Wnt/β-catenin signaling regulates pathogenesis of human middle ear cholesteatoma

Authors - Juan Lin
View Article
mouseIF

Front Pharmacol

Protective effect of Saussurea involucrata polysaccharide against skin dryness induced by ultraviolet radiation

Authors - Lusheng Wang
View Article