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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Cytokeratin 16 Polyclonal antibody

Cytokeratin 16 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17265

Synonyms

CK 16, CK16, Cytokeratin 16, K16, K1CP, keratin 16, KRT16, KRT16A, NEPPK

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated Cytokeratin 16 Polyc (CL488-17265)

    IF Staining of A431 using CL488-17265

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 Cytokeratin 16 antibody (CL488-17265) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17265 targets Cytokeratin 16 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 16 fusion protein Ag10999 相同性解析による交差性が予測される生物種
Full Name keratin 16
Calculated molecular weight 473 aa, 51 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC039169
Gene symbol Cytokeratin 16
Gene ID (NCBI) 3868
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 16 antibody CL488-17265Download protocol
Standard Protocols
Click here to view our Standard Protocols