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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 10712-1-AP

Synonyms

CK19, CK 19, KRT19, K19, Cytokeratin-19

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    WB analysis using 10712-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    WB analysis of A549 using 10712-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    WB analysis of A431 using 10712-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    WB analysis of HepG2 using 10712-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse placenta tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    WB analysis of mouse placenta using 10712-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    WB analysis of MCF-7 using 10712-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    WB analysis of mouse brain using 10712-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human colon using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human colon using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of mouse pancreas using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of mouse pancreas using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human breast cancer using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human breast cancer using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human thyroid cancer using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human thyroid cancer using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human pancreas using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human pancreas using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human oesophagus cancer using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human oesophagus cancer using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human thyroid cancer using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human thyroid cancer using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human stomach cancer using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human stomach cancer using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human skin using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human skin using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of mouse skin using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of mouse skin using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of mouse colon using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of mouse colon using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human lung cancer using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IHC staining of human lung cancer using 10712-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Retinal organoids using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IF Staining of Retinal organoids using 10712-1-AP

    Retinal organoids (day 60) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and Cytokeratin 19 using 10712-1-AP at 1:200 (red). Nuclear stain DAPI (blue). Scale bar = 100 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IF Staining of mouse pancreas using 10712-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IF Staining of mouse pancreas using 10712-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IF Staining of HeLa using 10712-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IF Staining of HeLa using 10712-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IF Staining of HaCaT using 10712-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    IF Staining of HepG2 using 10712-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of MCF-7 cells using Cytokeratin 19 Polyclonal antibody (10712-1-AP)

    FC experiment of MCF-7 using 10712-1-AP

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Cytokeratin 19 Polyclonal antibody (10712-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA431 cells, HepG2 cells, mouse placenta tissue, MCF-7 cells, A549 cells, mouse brain tissue, HT-29 cells
Positive IHC detected inhuman colon tissue, human breast cancer tissue, human lung cancer tissue, human oesophagus cancer tissue, human pancreas tissue, human skin tissue, human stomach cancer tissue, human thyroid cancer tissue, mouse colon tissue, mouse pancreas tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inRetinal organoids, mouse pancreas tissue
Positive IF/ICC detected inHaCaT cells, HeLa cells, HepG2 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:30000
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Immunofluorescence (IF)-PIF-P : 1:10-1:100
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10712-1-AP targets Cytokeratin 19 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig, goat, dog
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 19 fusion protein Ag1085 相同性解析による交差性が予測される生物種
Full Name keratin 19
Calculated molecular weight 40 kDa
Observed molecular weight 44-50 kDa
GenBank accession numberBC007628
Gene symbol Cytokeratin 19
Gene ID (NCBI) 3880
RRIDAB_2133325
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
IHC protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
IF protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

ACS Nano

Inflammation and Acinar Cell Dual-Targeting Nanomedicines for Synergistic Treatment of Acute Pancreatitis via Ca2+ Homeostasis Regulation and Pancreas Autodigestion Inhibition

Authors - Yanan Wang
View Article
mouseIHC,IF

Nat Commun

Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy

Authors - Xiaoyu Liu
View Article
humanIHC

Sci Transl Med

PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.

Authors - Tian-Yi Jiang
View Article
human

Adv Sci (Weinh)

PMID: 31728273

Authors - Qingming Tang
View Article
human

Nat Commun

Reversal of pancreatic desmoplasia by re-educating stellate cells with a tumour microenvironment-activated nanosystem.

Authors - Xuexiang Han
View Article
humanIHC

J Clin Invest

CCN1 induces hepatic ductular reaction through integrin αvβ5-mediated activation of NF-κB.

Authors - Ki-Hyun Kim
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Alessandro (Verified Customer) (07-27-2022)

No aspecific staining, great outcome

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: ROs