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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 555-conjugated Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-10712

Synonyms

CK19, CK 19, KRT19, K19, Cytokeratin-19

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 555-conjugated Cytokeratin 19 Polyc (CL555-10712)

    IF Staining of HeLa using CL555-10712

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 555 Cytokeratin 19 antibody (CL555-10712) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 555-conjugated Cytokeratin 19 Polyc (CL555-10712)

    FC experiment of A431 using CL555-10712

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 555 Anti-Human Cytokeratin 19 (CL555-10712) and HRP-conjugated polymer at dilution 1:1000 (red), or 0.4 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-10712 targets Cytokeratin 19 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 19 fusion protein Ag1085 相同性解析による交差性が予測される生物種
Full Name keratin 19
Calculated molecular weight 40 kDa
Observed molecular weight 44-50 kDa
GenBank accession numberBC007628
Gene symbol Cytokeratin 19
Gene ID (NCBI) 3880
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

Protocols

Product Specific Protocols
IF protocol for CL Plus 555 Cytokeratin 19 antibody CL555-10712Download protocol
FC protocol for CL Plus 555 Cytokeratin 19 antibody CL555-10712Download protocol
Standard Protocols
Click here to view our Standard Protocols