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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 20 Polyclonal antibody

Cytokeratin 20 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 17329-1-AP

Synonyms

KRT20, keratin 20, K20, Cytokeratin-20, CK-20

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    WB analysis using 17329-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    WB analysis of HeLa using 17329-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17329-1-AP (Cytokeratin 20 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IHC staining of human colon using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IHC staining of human colon using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IHC staining of human urothelial carcinoma using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IHC staining of human appendicitis using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IHC staining of human appendicitis using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IHC staining of human colon cancer using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IHC staining of human colon cancer using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IHC staining of human stomach cancer using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IHC staining of human stomach cancer using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IHC staining of human urothelial carcinoma using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IHC staining of human urothelial carcinoma using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IHC staining of human colon using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IHC staining of human colon using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IHC staining of human urothelial carcinoma using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Rabbit colon tissue using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IF Staining of Rabbit colon using 17329-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Rabbit colon tissue using Cytokeratin 20 antibody (17329-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Rabbit colon tissue using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IF Staining of Rabbit colon using 17329-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Rabbit colon tissue using Cytokeratin 20 antibody (17329-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IF Staining of Caco-2 using 17329-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Cytokeratin 20 antibody (17329-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    IF Staining of HeLa using 17329-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HT-29 cells using Cytokeratin 20 Polyclonal antibody (17329-1-AP)

    FC experiment of HT-29 using 17329-1-AP

    1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 20 (17329-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, HT-29 cells
Positive IHC detected inhuman colon tissue, human appendicitis tissue, human colon cancer tissue, human stomach cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inRabbit colon tissue
Positive IF/ICC detected inCaco-2 cells, HeLa cells
Positive FC (Intra) detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17329-1-AP targets Cytokeratin 20 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 20 fusion protein Ag11308 相同性解析による交差性が予測される生物種
Full Name keratin 20
Calculated molecular weight 424 aa, 48 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC031559
Gene symbol Cytokeratin 20
Gene ID (NCBI) 54474
RRIDAB_2133592
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 20 antibody 17329-1-APDownload protocol
IHC protocol for Cytokeratin 20 antibody 17329-1-APDownload protocol
IF protocol for Cytokeratin 20 antibody 17329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Res

Gut microbiota drives macrophage-dependent self-renewal of intestinal stem cells via niche enteric serotonergic neurons.

Authors - Pingping Zhu
View Article
mouseWB

Nat Cell Biol

LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.

Authors - Pingping Zhu
View Article

Neuron

5-hydroxytryptamine produced by enteric serotonergic neurons initiates colorectal cancer stem cell self-renewal and tumorigenesis.

Authors - Pingping Zhu
View Article
humanIF

Biomaterials

A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium.

Authors - Yuli Wang
View Article
mouseIF

Cancer Lett

LGR4 cooperates with PrPc to endow the stemness of colorectal cancer stem cells contributing to tumorigenesis and liver metastasis.

Authors - Qi Cheng
View Article
mouseIHC

Nutrients

Propolis Has an Anticancer Effect on Early Stage Colorectal Cancer by Affecting Epithelial Differentiation and Gut Immunity in the Tumor Microenvironment

Authors - Ming-Hung Shen
View Article