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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Cytokeratin 20 Polyclonal antibody

Cytokeratin 20 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, rabbit

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17329

Synonyms

KRT20, keratin 20, K20, Cytokeratin-20, CK-20

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using CoraLite® Plus 488-conjugated Cytokeratin 20 Polyc (CL488-17329)

    IF Staining of HT-29 using CL488-17329

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using CoraLite® Plus 488 Cytokeratin 20 antibody (CL488-17329) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HT-29 cells using CoraLite® Plus 488-conjugated Cytokeratin 20 Polyc (CL488-17329)

    FC experiment of HT-29 using CL488-17329

    1x10^6 HT-29 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Cytokeratin 20 Polyclonal antibody (CL488-17329)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHT-29 cells
Positive FC (Intra) detected inHT-29 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17329 targets Cytokeratin 20 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 20 fusion protein Ag11308 相同性解析による交差性が予測される生物種
Full Name keratin 20
Calculated molecular weight 424 aa, 48 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC031559
Gene symbol Cytokeratin 20
Gene ID (NCBI) 54474
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 20 antibody CL488-17329Download protocol
FC protocol for CL Plus 488 Cytokeratin 20 antibody CL488-17329Download protocol
Standard Protocols
Click here to view our Standard Protocols