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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Keratin 81 Polyclonal antibody

Keratin 81 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11342

Synonyms

ghHb1, ghHkb1, Hair keratin K2.9, Hb 1, HB1, hHAKB2 1, K81, keratin 81, Keratin, hair, basic, 1, Keratin, type II cuticular Hb1, KRT81, KRTHB1, MLN 137, MLN137, Type II hair keratin Hb1, Type II keratin Kb21

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated KRT81 Polyclonal ant (CL488-11342)

    IF Staining of HeLa using CL488-11342

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 KRT81 antibody (CL488-11342) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11342 targets Keratin 81 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Keratin 81 fusion protein Ag1890 相同性解析による交差性が予測される生物種
Full Name keratin 81
Calculated molecular weight 55 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC021241
Gene symbol KRT81
Gene ID (NCBI) 3887
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT81 is a type II keratin.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Keratin 81 antibody CL488-11342Download protocol
Standard Protocols
Click here to view our Standard Protocols