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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ki-67 Recombinant antibody

Ki-67 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241768E2

Cat no : 84432-1-RR

Synonyms

Mki67, Ki67, Ki 67, Proliferation related Ki 67 antigen, 241768E2

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Ki-67 Recombinant antibody (84432-1-RR)

    WB analysis of NIH/3T3 using 84432-1-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84432-1-RR (Ki67/MKI67 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Ki-67 Recombinant antibody (84432-1-RR)

    IHC staining of mouse spleen using 84432-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Ki-67 Recombinant antibody (84432-1-RR)

    IHC staining of mouse spleen using 84432-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat spleen tissue using Ki-67 Recombinant antibody (84432-1-RR)

    IHC staining of rat spleen using 84432-1-RR

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat spleen tissue using Ki-67 Recombinant antibody (84432-1-RR)

    IHC staining of rat spleen using 84432-1-RR

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using Ki-67 Recombinant antibody (84432-1-RR)

    IF Staining of C2C12 using 84432-1-RR

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84432-1-RR

    Affinity and Kinetic Characterization of 84432-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84432-1-RR against Mouse Ki-67 were performed. The affinity constant is 3.47 nM.

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Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IHC detected inmouse spleen tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84432-1-RR targets Ki-67 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name antigen identified by monoclonal antibody Ki 67
Calculated molecular weight351kDa
Observed molecular weight350 kDa
GenBank accession numberNM_001081117.2
Gene symbol ki67
Gene ID (NCBI) 17345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.

Protocols

Product Specific Protocols
WB protocol for Ki-67 antibody 84432-1-RRDownload protocol
IHC protocol for Ki-67 antibody 84432-1-RRDownload protocol
IF protocol for Ki-67 antibody 84432-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols