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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse Ki-67 Recombinant antibody, PBS Only (Detector)

Ki-67 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241768E2

Cat no : 84432-1-PBS

Synonyms

Mki67, Ki67, Ki 67, Proliferation related Ki 67 antigen, 241768E2

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Ki-67 Recombinant antibody (84432-1-RR)

    WB analysis of NIH/3T3 using 84432-1-RR (same clone as 84432-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84432-1-RR (Ki67/MKI67 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Ki-67 Recombinant antibody (84432-1-RR)

    IHC staining of mouse spleen using 84432-1-RR (same clone as 84432-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Ki-67 Recombinant antibody (84432-1-RR)

    IHC staining of mouse spleen using 84432-1-RR (same clone as 84432-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using Ki-67 Recombinant antibody (84432-1-RR)

    IHC staining of rat spleen using 84432-1-RR (same clone as 84432-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using Ki-67 Recombinant antibody (84432-1-RR)

    IHC staining of rat spleen using 84432-1-RR (same clone as 84432-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using Ki-67 Recombinant antibody (84432-1-RR)

    IF Staining of C2C12 using 84432-1-RR (same clone as 84432-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01277-1

    Cytometric bead array standard curve of MP01277-1

    Cytometric bead array standard curve of MP01277-1, MOUSE Ki67 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84432-2-PBS. Detection antibody: 84432-1-PBS. Standard: Eg1548. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 84432-1-RR

    Affinity and Kinetic Characterization of 84432-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84432-1-RR against Mouse Ki-67 were performed. The affinity constant is 3.47 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84432-1-PBS targets Ki-67 as part of a matched antibody pair:

MP01277-1: 84432-2-PBS capture and 84432-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name antigen identified by monoclonal antibody Ki 67
Calculated molecular weight351kDa
Observed molecular weight350 kDa
GenBank accession numberNM_001081117.2
Gene symbol ki67
Gene ID (NCBI) 17345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.