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Validation Data Gallery

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  • Western Blot (WB) analysis of rat heart tissue using Kir6.2 Polyclonal antibody (16920-1-AP)

    WB analysis of rat heart using 16920-1-AP (same clone as 16920-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 16920-1-AP (Kir6.2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16920-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using Kir6.2 Polyclonal antibody (16920-1-AP)

    WB analysis of rat skeletal muscle using 16920-1-AP (same clone as 16920-1-PBS)

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16920-1-AP (Kir6.2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16920-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Kir6.2 Polyclonal antibody (16920-1-AP)

    WB analysis of HepG2 using 16920-1-AP (same clone as 16920-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16920-1-AP (KCNJ11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16920-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Kir6.2 Polyclonal antibody (16920-1-AP)

    WB analysis of human heart using 16920-1-AP (same clone as 16920-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 16920-1-AP (KCNJ11 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16920-1-PBS in a different storage buffer formulation.

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Kir6.2 Polyclonal antibody, PBS Only

Kir6.2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 16920-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16920-1-PBS targets Kir6.2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kir6.2 fusion protein Ag10262 相同性解析による交差性が予測される生物種
Full Name potassium inwardly-rectifying channel, subfamily J, member 11
Calculated molecular weight 390 aa, 44 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC064497
Gene Symbol Kir6.2
Gene ID (NCBI) 3767
RRIDAB_2130461
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14654
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Kir6.2 (also known as BIR or IKATP), encoded by the KCNJ11 gene, is the pore-forming unit of the ATP-sensitive K+ channel, an inward-rectifier potassium ion channel. Kir6.2 is controlled by G-proteins and is found associated with the sulfonylurea receptor (SUR) to constitute the ATP-sensitive K+ channel. The KCNJ11 gene is located at 11p15.1 and has no intron. Mutations in KCNJ11 are a cause of familial PHHI, an autosomal recessive disorder characterized by unregulated ins secretion. Defects in KCNJ11 may also contribute to autosomal dominant non-ins-dependent diabetes mellitus type II (NIDDM), transient neonatal diabetes mellitus type 3 (TNDM3), and permanent neonatal diabetes mellitus (PNDM).