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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated L1CAM Recombinant antibody

L1CAM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240978A7

Cat no : CL488-84034-5

Synonyms

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite® Plus 488-conjugated L1CAM Recombinant an (CL488-84034-5)

    IF Staining of SH-SY5Y using CL488-84034-5

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 488 L1CAM antibody (CL488-84034-5, Clone: 240978A7 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84034-5 targets L1CAM in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name L1 cell adhesion molecule
Calculated molecular weight 1257 aa, 140 kDa
GenBank accession numberBC126229
Gene symbol L1CAM
Gene ID (NCBI) 3897
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 L1CAM antibody CL488-84034-5Download protocol
Standard Protocols
Click here to view our Standard Protocols