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Validation Data Gallery

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  • Western Blot (WB) analysis of NCCIT cells using L1TD1 Polyclonal antibody (21528-1-AP)

    WB analysis of NCCIT cells using 21528-1-AP (same clone as 21528-1-PBS)

    WB result of L1TD1 antibody (21528-1-AP, 1:500) with NCCIT cells. This data was developed using the same antibody clone with 21528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human placenta using 21528-1-AP (same clone as 21528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21528-1-AP (L1TD1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human placenta using 21528-1-AP (same clone as 21528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21528-1-AP (L1TD1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human ovary cancer using 21528-1-AP (same clone as 21528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 21528-1-AP (L1TD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human ovary cancer using 21528-1-AP (same clone as 21528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 21528-1-AP (L1TD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human ovary tumor using 21528-1-AP (same clone as 21528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21528-1-AP (L1TD1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human ovary tumor using 21528-1-AP (same clone as 21528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21528-1-AP (L1TD1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21528-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NCCIT cells using L1TD1 Polyclonal antibody (21528-1-AP)

    IF Staining of NCCIT using 21528-1-AP (same clone as 21528-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NCCIT cells using L1TD1 antibody (21528-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 21528-1-PBS in a different storage buffer formulation.

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L1TD1 Polyclonal antibody, PBS Only

L1TD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21528-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

LINE-1 type transposase domain-containing protein 1, ES cell-associated protein 11, ES cell associated protein 11, ECAT11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21528-1-PBS targets L1TD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen L1TD1 fusion protein Ag16147 相同性解析による交差性が予測される生物種
Full Name LINE-1 type transposase domain containing 1
Calculated molecular weight 865 aa, 99 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC111559
Gene Symbol L1TD1
Gene ID (NCBI) 54596
RRIDAB_2878878
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T7N2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

L1TD1 is an RNA-binding protein highly and specifically expressed in pluripotent cells. It is essential for maintaining the pluripotentency in human embryonic stem cells (hESCs) and has been considered as a marker for undifferentiated hESCs. L1TD1 is also reported to be highly expressed in medulloblastoma cells and its expression in medulloblastoma correlates with poor clinical outcome.