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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

L1TD1 Polyclonal antibody, PBS Only

L1TD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21528-1-PBS

Synonyms

LINE-1 type transposase domain-containing protein 1, ES cell-associated protein 11, ES cell associated protein 11, ECAT11

Validation Data Gallery

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  • Western Blot (WB) analysis of NCCIT cells using L1TD1 Polyclonal antibody (21528-1-AP)

    WB analysis of NCCIT cells using 21528-1-AP (same clone as 21528-1-PBS)

    WB result of L1TD1 antibody (21528-1-AP, 1:500) with NCCIT cells. This data was developed using the same antibody clone with 21528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human placenta using 21528-1-AP (same clone as 21528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21528-1-AP (L1TD1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human placenta using 21528-1-AP (same clone as 21528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21528-1-AP (L1TD1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human ovary cancer using 21528-1-AP (same clone as 21528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 21528-1-AP (L1TD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human ovary cancer using 21528-1-AP (same clone as 21528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 21528-1-AP (L1TD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human ovary tumor using 21528-1-AP (same clone as 21528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21528-1-AP (L1TD1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human ovary tumor using 21528-1-AP (same clone as 21528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21528-1-AP (L1TD1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21528-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NCCIT cells using L1TD1 Polyclonal antibody (21528-1-AP)

    IF Staining of NCCIT using 21528-1-AP (same clone as 21528-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NCCIT cells using L1TD1 antibody (21528-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 21528-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21528-1-PBS targets L1TD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen L1TD1 fusion protein Ag16147 相同性解析による交差性が予測される生物種
Full Name LINE-1 type transposase domain containing 1
Calculated molecular weight 865 aa, 99 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC111559
Gene symbol L1TD1
Gene ID (NCBI) 54596
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

L1TD1 is an RNA-binding protein highly and specifically expressed in pluripotent cells. It is essential for maintaining the pluripotentency in human embryonic stem cells (hESCs) and has been considered as a marker for undifferentiated hESCs. L1TD1 is also reported to be highly expressed in medulloblastoma cells and its expression in medulloblastoma correlates with poor clinical outcome.