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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using L2HGDH Polyclonal antibody (15707-1-AP)

    WB analysis using 15707-1-AP (same clone as 15707-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using L2HGDH Polyclonal antibody (15707-1-AP)

    WB analysis using 15707-1-AP (same clone as 15707-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using L2HGDH Polyclonal antibody (15707-1-AP)

    WB analysis of mouse small intestine using 15707-1-AP (same clone as 15707-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SGC-7901 cells using L2HGDH Polyclonal antibody (15707-1-AP)

    WB analysis of SGC-7901 using 15707-1-AP (same clone as 15707-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using L2HGDH Polyclonal antibody (15707-1-AP)

    WB analysis of rat brain using 15707-1-AP (same clone as 15707-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH Antibody) at dilution of 1:800 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using L2HGDH Polyclonal antibody (15707-1-AP)

    IHC staining of human liver cancer using 15707-1-AP (same clone as 15707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using L2HGDH Polyclonal antibody (15707-1-AP)

    IHC staining of human liver cancer using 15707-1-AP (same clone as 15707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using L2HGDH Polyclonal antibody (15707-1-AP)

    IHC staining of human gliomas using 15707-1-AP (same clone as 15707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using L2HGDH Polyclonal antibody (15707-1-AP)

    IHC staining of human gliomas using 15707-1-AP (same clone as 15707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

L2HGDH Polyclonal antibody, PBS Only

L2HGDH Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15707-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15707-1-PBS targets L2HGDH in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen L2HGDH fusion protein Ag8382 相同性解析による交差性が予測される生物種
Full Name L-2-hydroxyglutarate dehydrogenase
Calculated molecular weight463aa,50 kDa; 441aa,49 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC006117
Gene Symbol L2HGDH
Gene ID (NCBI) 79944
RRIDAB_2133202
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H9P8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family. The putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa. L2HGDH also can be detected as ~45kD due to the 51aa transit peptide cleaved.