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Validation Data Gallery

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  • Western Blot (WB) analysis of pig brain tissue using L2HGDH Monoclonal antibody (66269-1-Ig)

    WB analysis of pig brain using 66269-1-Ig (same clone as 66269-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66269-1-Ig (L2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using L2HGDH Monoclonal antibody (66269-1-Ig)

    WB analysis of MCF-7 using 66269-1-Ig (same clone as 66269-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66269-1-Ig (L2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using L2HGDH Monoclonal antibody (66269-1-Ig)

    IHC staining of human breast cancer using 66269-1-Ig (same clone as 66269-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66269-1-Ig (L2HGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using L2HGDH Monoclonal antibody (66269-1-Ig)

    IHC staining of human breast cancer using 66269-1-Ig (same clone as 66269-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66269-1-Ig (L2HGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using L2HGDH Monoclonal antibody (66269-1-Ig)

    IF Staining of human breast cancer using 66269-1-Ig (same clone as 66269-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using L2HGDH antibody (66269-1-Ig, Clone: 3D11C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using L2HGDH Monoclonal antibody (66269-1-Ig)

    IF Staining of human breast cancer using 66269-1-Ig (same clone as 66269-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using L2HGDH antibody (66269-1-Ig, Clone: 3D11C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

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L2HGDH Monoclonal antibody, PBS Only

L2HGDH Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66269-1-PBS
Clone No.3D11C2

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

C14orf160, DURANIN, L2HGDH

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66269-1-PBS targets L2HGDH in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen L2HGDH fusion protein Ag8382 相同性解析による交差性が予測される生物種
Full Name L-2-hydroxyglutarate dehydrogenase
Calculated molecular weight463aa,50 kDa; 441aa,49 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC006117
Gene Symbol L2HGDH
Gene ID (NCBI) 79944
RRIDAB_2881654
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H9P8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family. The putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa. L2HGDH also can be detected as ~45kD due to the 51aa transit peptide cleaved.