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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

L2HGDH Monoclonal antibody

L2HGDH Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3D11C2

Cat no : 66269-1-Ig

Synonyms

C14orf160, DURANIN, L2HGDH

Validation Data Gallery

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  • Western Blot (WB) analysis of pig brain tissue using L2HGDH Monoclonal antibody (66269-1-Ig)

    WB analysis of pig brain using 66269-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66269-1-Ig (L2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using L2HGDH Monoclonal antibody (66269-1-Ig)

    WB analysis of MCF-7 using 66269-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66269-1-Ig (L2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using L2HGDH Monoclonal antibody (66269-1-Ig)

    IHC staining of human breast cancer using 66269-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66269-1-Ig (L2HGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using L2HGDH Monoclonal antibody (66269-1-Ig)

    IHC staining of human breast cancer using 66269-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66269-1-Ig (L2HGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using L2HGDH Monoclonal antibody (66269-1-Ig)

    IF Staining of human breast cancer using 66269-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using L2HGDH antibody (66269-1-Ig, Clone: 3D11C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using L2HGDH Monoclonal antibody (66269-1-Ig)

    IF Staining of human breast cancer using 66269-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using L2HGDH antibody (66269-1-Ig, Clone: 3D11C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inpig brain tissue, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66269-1-Ig targets L2HGDH in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen L2HGDH fusion protein Ag8382 相同性解析による交差性が予測される生物種
Full Name L-2-hydroxyglutarate dehydrogenase
Calculated molecular weight463aa,50 kDa; 441aa,49 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC006117
Gene symbol L2HGDH
Gene ID (NCBI) 79944
RRIDAB_2881654
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family. The putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa. L2HGDH also can be detected as ~45kD due to the 51aa transit peptide cleaved.

Protocols

Product Specific Protocols
WB protocol for L2HGDH antibody 66269-1-IgDownload protocol
IHC protocol for L2HGDH antibody 66269-1-IgDownload protocol
IF protocol for L2HGDH antibody 66269-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols