CoraLite® Plus 488-conjugated L2HGDH Monoclonal antibody

L2HGDH Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3D11C2

Cat no : CL488-66269

Synonyms

C14orf160, DURANIN, FLJ12618, L2HGDH


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Tested Applications

Positive IF-P detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66269 targets L2HGDH in IF-P applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen L2HGDH fusion protein Ag8382 相同性解析による交差性が予測される生物種
Full Name L-2-hydroxyglutarate dehydrogenase
Calculated molecular weight463aa,50 kDa; 441aa,49 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC006117
Gene symbol L2HGDH
Gene ID (NCBI) 79944
RRIDAB_2919308
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family. The putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa. L2HGDH also can be detected as ~45kD due to the 51aa transit peptide cleaved.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 L2HGDH antibody CL488-66269Download protocol
Standard Protocols
Click here to view our Standard Protocols