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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using LACTB2 Monoclonal antibody (67399-1-Ig)

    WB analysis of A549 using 67399-1-Ig

    WB result of LACTB2 antibody (67399-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LACTB2 transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using LACTB2 Monoclonal antibody (67399-1-Ig)

    WB analysis using 67399-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67399-1-Ig (LACTB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using LACTB2 Monoclonal antibody (67399-1-Ig)

    WB analysis of NIH/3T3 using 67399-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67399-1-Ig (LACTB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LACTB2 Monoclonal antibody (67399-1-Ig)

    IHC staining of human liver cancer using 67399-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67399-1-Ig (LACTB2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LACTB2 Monoclonal antibody (67399-1-Ig)

    IHC staining of human liver cancer using 67399-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67399-1-Ig (LACTB2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using LACTB2 Monoclonal antibody (67399-1-Ig)

    IF Staining of human liver cancer using 67399-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using LACTB2 antibody (67399-1-Ig, Clone: 1G6B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using LACTB2 Monoclonal antibody (67399-1-Ig)

    IF Staining of human liver cancer using 67399-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using LACTB2 antibody (67399-1-Ig, Clone: 1G6B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using LACTB2 Monoclonal antibody (67399-1-Ig)

    IF Staining of mouse liver using 67399-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using LACTB2 antibody (67399-1-Ig, Clone: 1G6B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using LACTB2 Monoclonal antibody (67399-1-Ig)

    IF Staining of A549 using 67399-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using LACTB2 antibody (67399-1-Ig, Clone: 1G6B1 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004).

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  • KD/KO Validated

LACTB2 Monoclonal antibody

LACTB2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 67399-1-Ig
Clone No.1G6B1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

EC:3.1.27.-, CGI-83, CGI83, CGI 83, Beta-lactamase-like protein 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue, mouse liver tissue
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67399-1-Ig targets LACTB2 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LACTB2 fusion protein Ag10231 相同性解析による交差性が予測される生物種
Full Name lactamase, beta 2
Calculated molecular weight 288 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC000878
Gene Symbol LACTB2
Gene ID (NCBI) 51110
RRIDAB_2882642
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ53H82
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LACTB2 antibody 67399-1-IgDownload protocol
IHC protocol for LACTB2 antibody 67399-1-IgDownload protocol
IF protocol for LACTB2 antibody 67399-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols