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  • Featured Product
  • KD/KO Validated

LACTB2 Monoclonal antibody, PBS Only (Capture)

LACTB2 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G6B1

Cat no : 67399-1-PBS

Synonyms

EC:3.1.27.-, CGI-83, CGI83, CGI 83, Beta-lactamase-like protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using LACTB2 Monoclonal antibody (67399-1-Ig)

    WB analysis of A549 using 67399-1-Ig (same clone as 67399-1-PBS)

    WB result of LACTB2 antibody (67399-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LACTB2 transfected A549 cells. This data was developed using the same antibody clone with 67399-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LACTB2 Monoclonal antibody (67399-1-Ig)

    WB analysis using 67399-1-Ig (same clone as 67399-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67399-1-Ig (LACTB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67399-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using LACTB2 Monoclonal antibody (67399-1-Ig)

    WB analysis of NIH/3T3 using 67399-1-Ig (same clone as 67399-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67399-1-Ig (LACTB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67399-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LACTB2 Monoclonal antibody (67399-1-Ig)

    IHC staining of human liver cancer using 67399-1-Ig (same clone as 67399-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67399-1-Ig (LACTB2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67399-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LACTB2 Monoclonal antibody (67399-1-Ig)

    IHC staining of human liver cancer using 67399-1-Ig (same clone as 67399-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67399-1-Ig (LACTB2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67399-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using LACTB2 Monoclonal antibody (67399-1-Ig)

    IF Staining of human liver cancer using 67399-1-Ig (same clone as 67399-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using LACTB2 antibody (67399-1-Ig, Clone: 1G6B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67399-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using LACTB2 Monoclonal antibody (67399-1-Ig)

    IF Staining of human liver cancer using 67399-1-Ig (same clone as 67399-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using LACTB2 antibody (67399-1-Ig, Clone: 1G6B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67399-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50726-4

    Cytometric bead array standard curve of MP50726-4

    Cytometric bead array standard curve of MP50726-4, LACTB2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67399-1-PBS. Detection antibody: 67399-4-PBS. Standard:Ag10231. Range: 3.125-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67399-1-PBS targets LACTB2 as part of a matched antibody pair:

MP50726-4: 67399-1-PBS capture and 67399-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LACTB2 fusion protein Ag10231 相同性解析による交差性が予測される生物種
Full Name lactamase, beta 2
Calculated molecular weight 288 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC000878
Gene symbol LACTB2
Gene ID (NCBI) 51110
RRIDAB_2882642
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.