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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAMP5 Recombinant antibody (82992-2-RR)

    WB analysis using 82992-2-RR (same clone as 82992-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82992-2-RR (LAMP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82992-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LAMP5 Recombinant antibody (82992-2-RR)

    WB analysis using 82992-2-RR (same clone as 82992-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82992-2-RR (LAMP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82992-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using LAMP5 Recombinant antibody (82992-2-RR)

    IF Staining of Neuro-2a using 82992-2-RR (same clone as 82992-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using LAMP5 antibody (82992-2-RR, Clone: 230102F12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82992-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using LAMP5 Recombinant antibody (82992-2-RR)

    IF Staining of SH-SY5Y using 82992-2-RR (same clone as 82992-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using LAMP5 antibody (82992-2-RR, Clone: 230102F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82992-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using LAMP5 Recombinant antibody (82992-2-RR)

    FC experiment of SH-SY5Y using 82992-2-RR (same clone as 82992-2-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug LAMP5 Recombinant antibody (82992-2-RR, Clone:230102F12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82992-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00069-3

    Cytometric bead array standard curve of MP00069-3

    Cytometric bead array standard curve of MP00069-3, LAMP5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82992-4-PBS. Detection antibody: 82992-2-PBS. Standard: Ag33894. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 82992-2-RR

    Affinity and Kinetic Characterization of 82992-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82992-2-RR against Human LAMP5 were performed. The affinity constant is 0.247 nM.

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LAMP5 Recombinant antibody, PBS Only (Detector)

LAMP5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 82992-2-PBS
Clone No.230102F12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

C20orf103, LAMP-5, Brain-associated LAMP-like protein, Brain and dendritic cell-associated LAMP, BAD-LAMP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82992-2-PBS targets LAMP5 as part of a matched antibody pair:

MP00069-3: 82992-4-PBS capture and 82992-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LAMP5 fusion protein Ag33894 相同性解析による交差性が予測される生物種
Full Name chromosome 20 open reading frame 103
Observed molecular weight 31-35 kDa
GenBank accession numberBC050727
Gene Symbol LAMP5
Gene ID (NCBI) 24141
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UJQ1
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

LAMP5 (Lysosome-associated membrane glycoprotein 5) is also named as BAD-LAMP and C20orf103. BAD-LAMP is a novel biomarker of nonactivated human plasmacytoid dendritic cells (PMID: 21642595). LAMP5 may be one of the key genes in the development of Multiple myeloma (MM) as well as its recurrence  (PMID: 37033323). LAMP5 is a mammalian ortholog of the Caenorhabditis elegans protein, UNC-46, which functions as a sorting factor to localize the vesicular GABA transporter UNC-47 to synaptic vesicles (PMID: 30867010). It was localized exclusively in inhibitory synaptic terminals (PMID: 30867010).