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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LASP1 Recombinant antibody (82491-1-RR)

    WB analysis using 82491-1-RR (same clone as 82491-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82491-1-RR (LASP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82491-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using LASP1 Recombinant antibody (82491-1-RR)

    IHC staining of human stomach using 82491-1-RR (same clone as 82491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82491-1-RR (LASP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82491-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using LASP1 Recombinant antibody (82491-1-RR)

    IHC staining of human colon using 82491-1-RR (same clone as 82491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 82491-1-RR (LASP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82491-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LASP1 Recombinant antibody (82491-1-RR)

    IF Staining of HeLa using 82491-1-RR (same clone as 82491-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LASP1 antibody (82491-1-RR, Clone: 4J2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82491-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82491-1-RR

    Affinity and Kinetic Characterization of 82491-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82491-1-RR against Human LASP1 were performed. The affinity constant is 10.3 nM.

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LASP1 Recombinant antibody, PBS Only

LASP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82491-1-PBS
Clone No.4J2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MLN50, LIM and SH3 protein 1, LASP-1, 4J2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82491-1-PBS targets LASP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LASP1 fusion protein Ag0800 相同性解析による交差性が予測される生物種
Full Name LIM and SH3 protein 1
Calculated molecular weight 30 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC012460
Gene Symbol LASP1
Gene ID (NCBI) 3927
ENSEMBL Gene IDENSG00000002834
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14847
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

LASP1(LIM and SH3 protein 1), also known as MLN50, is a 261 amino acid protein that localizes to both the cytoplasm and the cytoskeleton(PMID: 7589475). LASP1 consists of an N-terminal LIM-domain with two zinc finger motifs, followed by two central actin-binding nebulin repeats, flanked by a linker region and a C-terminal SH3 domain (PMID: 17177073, 9848085). LASP-1 interacts with F-Actin and plays an important role in the regulation of Actin-associated cytoskeletal organization. Agonist-dependent changes in LASP1 phosphorylation may regulate Actin-related ion transport activities in epithelial cells (PMID: 15465019,12571245). Overexpression of LASP-1 is associated with breast cancer, and plays a role in tumor transformation and metastasis (PMID: 17956604).