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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LASP1 Recombinant antibody

LASP1 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4J2

Cat no : 82491-1-RR

Synonyms

MLN50, LIM and SH3 protein 1, LASP-1, 4J2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LASP1 Recombinant antibody (82491-1-RR)

    WB analysis using 82491-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82491-1-RR (LASP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LASP1 Recombinant antibody (82491-1-RR)

    IF Staining of HeLa using 82491-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LASP1 antibody (82491-1-RR, Clone: 4J2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82491-1-RR

    Affinity and Kinetic Characterization of 82491-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82491-1-RR against Human LASP1 were performed. The affinity constant is 10.3 nM.

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Tested Applications

Positive WB detected inA431 cells, SKOV-3 cells, PC-3 cells, HCT 116 cells, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82491-1-RR targets LASP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LASP1 fusion protein Ag0800 相同性解析による交差性が予測される生物種
Full Name LIM and SH3 protein 1
Calculated molecular weight 30 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC012460
Gene symbol LASP1
Gene ID (NCBI) 3927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LASP1(LIM and SH3 protein 1), also known as MLN50, is a 261 amino acid protein that localizes to both the cytoplasm and the cytoskeleton(PMID: 7589475). LASP1 consists of an N-terminal LIM-domain with two zinc finger motifs, followed by two central actin-binding nebulin repeats, flanked by a linker region and a C-terminal SH3 domain (PMID: 17177073, 9848085). LASP-1 interacts with F-Actin and plays an important role in the regulation of Actin-associated cytoskeletal organization. Agonist-dependent changes in LASP1 phosphorylation may regulate Actin-related ion transport activities in epithelial cells (PMID: 15465019,12571245). Overexpression of LASP-1 is associated with breast cancer, and plays a role in tumor transformation and metastasis (PMID: 17956604).

Protocols

Product Specific Protocols
WB protocol for LASP1 antibody 82491-1-RRDownload protocol
IF protocol for LASP1 antibody 82491-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols