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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LC3B-Specific Polyclonal antibody, PBS Only

LC3B-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 18725-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human brain tissue using LC3B-Specific Polyclonal antibody (18725-1-AP)

    WB analysis of human brain using 18725-1-AP (same clone as 18725-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of TN treated Hela using LC3B-Specific Polyclonal antibody (18725-1-AP)

    WB analysis of TN treated Hela using 18725-1-AP (same clone as 18725-1-PBS)

    TN treated Hela cells were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using LC3B-Specific Polyclonal antibody (18725-1-AP)

    WB analysis of A549 using 18725-1-AP (same clone as 18725-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LC3B-Specific Polyclonal antibody (18725-1-AP)

    WB analysis using 18725-1-AP (same clone as 18725-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using LC3B-Specific Polyclonal antibody (18725-1-AP)

    WB analysis of MCF-7 using 18725-1-AP (same clone as 18725-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using LC3B-Specific Polyclonal antibody (18725-1-AP)

    WB analysis of mouse brain using 18725-1-AP (same clone as 18725-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of UV treated HEK-293 using LC3B-Specific Polyclonal antibody (18725-1-AP)

    WB analysis of UV treated HEK-293 using 18725-1-AP (same clone as 18725-1-PBS)

    UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LC3B-Specific Polyclonal antibody (18725-1-AP)

    IHC staining of mouse brain using 18725-1-AP (same clone as 18725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LC3B-Specific Polyclonal antibody (18725-1-AP)

    IHC staining of mouse brain using 18725-1-AP (same clone as 18725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LC3B-Specific Polyclonal antibody (18725-1-AP)

    IHC staining of mouse brain using 18725-1-AP (same clone as 18725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using LC3B-Specific Polyclonal antibody (18725-1-AP)

    IHC staining of rat brain using 18725-1-AP (same clone as 18725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using LC3B-Specific Polyclonal antibody (18725-1-AP)

    IHC staining of rat brain using 18725-1-AP (same clone as 18725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using LC3B-Specific Polyclonal antibody (18725-1-AP)

    IHC staining of rat brain using 18725-1-AP (same clone as 18725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using LC3B-Specific Polyclonal antibody (18725-1-AP)

    IHC staining of rat brain using 18725-1-AP (same clone as 18725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LC3B-Specific Polyclonal antibody (18725-1-AP)

    IHC staining of mouse brain using 18725-1-AP (same clone as 18725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LC3B-Specific Polyclonal antibody (18725-1-AP)

    IF Staining of HeLa using 18725-1-AP (same clone as 18725-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using LC3B-Specific antibody (18725-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LC3B-Specific Polyclonal antibody (18725-1-AP)

    IF Staining of HepG2 using 18725-1-AP (same clone as 18725-1-PBS)

    Immunofluorescent analysis of -20℃ Ethanol fixed CQ treated HepG2 cells (left) and untreated HepG2 cells (right) using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton was labelled in red with 66031-1-Ig (alpha tubulin). This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LC3B-Specific Polyclonal antibody (18725-1-AP)

    IF Staining of HepG2 using 18725-1-AP (same clone as 18725-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LC3B-Specific Polyclonal antibody (18725-1-AP)

    IF Staining of HepG2 using 18725-1-AP (same clone as 18725-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed CQ treated HepG2 cells using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using LC3B-Specific Polyclonal antibody (18725-1-AP)

    FC experiment of HeLa using 18725-1-AP (same clone as 18725-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human LC3B-Specific (18725-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18725-1-PBS targets LC3B-Specific in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein 1 light chain 3 beta
Calculated molecular weight 15 kDa
Observed molecular weight 15 kDa, 18 kDa
GenBank accession numberNM_022818
Gene symbol LC3B
Gene ID (NCBI) 81631
RRIDAB_2137745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LC3B, also named as MAP1LC3B, MAP1A/1BLC3, belongs to the MAP1 LC3 family. It is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. In cell biology, autophagy, or autophagocytosis, is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery. It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Two forms of LC3, called LC3-I (17-19kd) and -II(14-16kd), were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. MAP1LC3 has 3 isoforms MAP1LC3A, MAP1LC3B and MAP1LC3C. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, like their rat counterpart, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form. This antibody is specific to LC3B.