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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LCK Monoclonal antibody, PBS Only (Capture)

LCK Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D10C2

Cat no : 60162-1-PBS

Synonyms

Lymphocyte cell-specific protein-tyrosine kinase, LSK, Leukocyte C-terminal Src kinase, Lck p56, EC:2.7.10.2

Validation Data Gallery

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  • Western Blot (WB) analysis of pig thymus tissue using LCK Monoclonal antibody (60162-1-Ig)

    WB analysis of pig thymus using 60162-1-Ig (same clone as 60162-1-PBS)

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LCK Monoclonal antibody (60162-1-Ig)

    WB analysis using 60162-1-Ig (same clone as 60162-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat thymus tissue using LCK Monoclonal antibody (60162-1-Ig)

    WB analysis of rat thymus using 60162-1-Ig (same clone as 60162-1-PBS)

    rat thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using LCK Monoclonal antibody (60162-1-Ig)

    WB analysis of Jurkat using 60162-1-Ig (same clone as 60162-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using LCK Monoclonal antibody (60162-1-Ig)

    WB analysis of Ramos using 60162-1-Ig (same clone as 60162-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LCK Monoclonal antibody (60162-1-Ig)

    IHC staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LCK Monoclonal antibody (60162-1-Ig)

    IHC staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using LCK Monoclonal antibody (60162-1-Ig)

    IHC staining of human lymphoma using 60162-1-Ig (same clone as 60162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using LCK Monoclonal antibody (60162-1-Ig)

    IHC staining of human lymphoma using 60162-1-Ig (same clone as 60162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using LCK Monoclonal antibody (60162-1-Ig)

    IF Staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using LCK Monoclonal antibody (60162-1-Ig)

    IF Staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LCK Monoclonal antibody (60162-1-Ig)

    IF Staining of HepG2 using 60162-1-Ig (same clone as 60162-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LCK Monoclonal antibody (60162-1-Ig)

    IF Staining of HepG2 using 60162-1-Ig (same clone as 60162-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using LCK Monoclonal antibody (60162-1-Ig)

    FC experiment of HepG2 using 60162-1-Ig (same clone as 60162-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human LCK (60162-1-Ig, Clone:2D10C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51238-1

    Cytometric bead array standard curve of MP51238-1

    Cytometric bead array standard curve of MP51238-1, LCK Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60162-1-PBS. Detection antibody: 60162-3-PBS. Standard:Ag7694. Range: 0.098-25 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60162-1-PBS targets LCK as part of a matched antibody pair:

MP51238-1: 60162-1-PBS capture and 60162-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LCK fusion protein Ag7694 相同性解析による交差性が予測される生物種
Full Name lymphocyte-specific protein tyrosine kinase
Calculated molecular weight 539 aa, 56 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC013200
Gene symbol LCK
Gene ID (NCBI) 3932
RRIDAB_10663101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.