LCK Monoclonal antibody, PBS Only

Name: LCK Monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 60162-1-PBS

LCK Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D10C2

Review Product

Cat no : 60162-1-PBS

Synonyms

Lymphocyte cell-specific protein-tyrosine kinase, LSK, Leukocyte C-terminal Src kinase, Lck p56, EC:2.7.10.2

Validation Data Gallery

Filter:

AllWBIHCIF-PIF/ICCFC (Intra)

WB analysis of pig thymus using 60162-1-Ig (same clone as 60162-1-PBS)
pig thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.
WB analysis using 60162-1-Ig (same clone as 60162-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.
WB analysis of rat thymus using 60162-1-Ig (same clone as 60162-1-PBS)
rat thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 60162-1-Ig (same clone as 60162-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.
WB analysis of Ramos using 60162-1-Ig (same clone as 60162-1-PBS)
Ramos cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 60162-1-Ig (same clone as 60162-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 60162-1-Ig (same clone as 60162-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 60162-1-Ig (same clone as 60162-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 60162-1-Ig (same clone as 60162-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 60162-1-Ig (same clone as 60162-1-PBS)
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human LCK (60162-1-Ig, Clone:2D10C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.
×
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human LCK (60162-1-Ig, Clone:2D10C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

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Cat No. 60162-1-PBS

Size/Concentration

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Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60162-1-PBS targets LCK in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity	human, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	LCK fusion protein Ag7694 相同性解析による交差性が予測される生物種
Full Name	lymphocyte-specific protein tyrosine kinase
Calculated molecular weight	539 aa, 56 kDa
Observed molecular weight	50-60 kDa
GenBank accession number	BC013200
Gene symbol	LCK
Gene ID (NCBI)	3932
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LCK Monoclonal antibody, PBS Only

LCK Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Validation Data Gallery

WB analysis of pig thymus using 60162-1-Ig (same clone as 60162-1-PBS)

WB analysis using 60162-1-Ig (same clone as 60162-1-PBS)

WB analysis of rat thymus using 60162-1-Ig (same clone as 60162-1-PBS)

WB analysis of Jurkat using 60162-1-Ig (same clone as 60162-1-PBS)

WB analysis of Ramos using 60162-1-Ig (same clone as 60162-1-PBS)

IHC staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

IHC staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

IHC staining of human lymphoma using 60162-1-Ig (same clone as 60162-1-PBS)