LCK Monoclonal antibody
LCK Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, pig
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
2D10C2
Cat no : 60162-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | NK-92 cells, Jurkat cells, pig thymus tissue, Ramos cells, rat thymus tissue, MOLT-4 cells, MJ cells |
Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
IHC | See 1 publications below |
CoIP | See 1 publications below |
Product Information
60162-1-Ig targets LCK in WB, IHC, IF/ICC, IF-P, FC (Intra), CoIP, ELISA applications and shows reactivity with human, rat, pig samples.
Tested Reactivity | human, rat, pig |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | LCK fusion protein Ag7694 相同性解析による交差性が予測される生物種 |
Full Name | lymphocyte-specific protein tyrosine kinase |
Calculated molecular weight | 539 aa, 56 kDa |
Observed molecular weight | 50-60 kDa |
GenBank accession number | BC013200 |
Gene symbol | LCK |
Gene ID (NCBI) | 3932 |
RRID | AB_10663101 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for LCK antibody 60162-1-Ig | Download protocol |
IHC protocol for LCK antibody 60162-1-Ig | Download protocol |
IF protocol for LCK antibody 60162-1-Ig | Download protocol |
FC protocol for LCK antibody 60162-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Int J Gen Med Identification of Biomarkers Related to Regulatory T Cell Infiltration in Oral Squamous Cell Carcinoma Based on Integrated Bioinformatics Analysis. | ||
Mol Med c-CBL/LCK/c-JUN/ETS1/CD28 axis restrains childhood asthma by suppressing Th2 differentiation |