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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LCK Monoclonal antibody

LCK Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2D10C2

Cat no : 60162-1-Ig

Synonyms

Lymphocyte cell-specific protein-tyrosine kinase, LSK, Leukocyte C-terminal Src kinase, Lck p56, EC:2.7.10.2

Validation Data Gallery

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  • Western Blot (WB) analysis of pig thymus tissue using LCK Monoclonal antibody (60162-1-Ig)

    WB analysis of pig thymus using 60162-1-Ig

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LCK Monoclonal antibody (60162-1-Ig)

    WB analysis using 60162-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat thymus tissue using LCK Monoclonal antibody (60162-1-Ig)

    WB analysis of rat thymus using 60162-1-Ig

    rat thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LCK Monoclonal antibody (60162-1-Ig)

    WB analysis of Jurkat using 60162-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ramos cells using LCK Monoclonal antibody (60162-1-Ig)

    WB analysis of Ramos using 60162-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LCK Monoclonal antibody (60162-1-Ig)

    IHC staining of human tonsillitis using 60162-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LCK Monoclonal antibody (60162-1-Ig)

    IHC staining of human tonsillitis using 60162-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using LCK Monoclonal antibody (60162-1-Ig)

    IHC staining of human lymphoma using 60162-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using LCK Monoclonal antibody (60162-1-Ig)

    IHC staining of human lymphoma using 60162-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using LCK Monoclonal antibody (60162-1-Ig)

    IF Staining of human tonsillitis using 60162-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using LCK Monoclonal antibody (60162-1-Ig)

    IF Staining of human tonsillitis using 60162-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LCK Monoclonal antibody (60162-1-Ig)

    IF Staining of HepG2 using 60162-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LCK Monoclonal antibody (60162-1-Ig)

    IF Staining of HepG2 using 60162-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using LCK Monoclonal antibody (60162-1-Ig)

    FC experiment of HepG2 using 60162-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human LCK (60162-1-Ig, Clone:2D10C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inNK-92 cells, Jurkat cells, pig thymus tissue, Ramos cells, rat thymus tissue, MOLT-4 cells, MJ cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60162-1-Ig targets LCK in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LCK fusion protein Ag7694 相同性解析による交差性が予測される生物種
Full Name lymphocyte-specific protein tyrosine kinase
Calculated molecular weight 539 aa, 56 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC013200
Gene symbol LCK
Gene ID (NCBI) 3932
RRIDAB_10663101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for LCK antibody 60162-1-IgDownload protocol
IHC protocol for LCK antibody 60162-1-IgDownload protocol
IF protocol for LCK antibody 60162-1-IgDownload protocol
FC protocol for LCK antibody 60162-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Int J Gen Med

Identification of Biomarkers Related to Regulatory T Cell Infiltration in Oral Squamous Cell Carcinoma Based on Integrated Bioinformatics Analysis.

Authors - Chao Wang
View Article