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Validation Data Gallery

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  • Flow cytometry (FC) experiment of A431 cells using CoraLite®594-conjugated LCK Monoclonal antibody (CL594-60162)

    FC experiment of A431 using CL594-60162

    1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human LCK (CL594-60162, Clone:2D10C2) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite®594-conjugated LCK Monoclonal antibody

LCK Monoclonal Antibody for FC (Intra)
Cat No. CL594-60162
Clone No.2D10C2

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

FC (Intra)

Synonyms

LCK, Lck p56, Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, p56-LCK, Proto-oncogene Lck, T cell-specific protein-tyrosine kinase, Tyrosine-protein kinase Lck, YT 16, YT16, 2D10C2, EC:2.7.10.2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60162 targets LCK in FC (Intra) applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LCK fusion protein Ag7694 相同性解析による交差性が予測される生物種
Full Name lymphocyte-specific protein tyrosine kinase
Calculated molecular weight 539 aa, 56 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC013200
Gene Symbol LCK
Gene ID (NCBI) 3932
RRIDAB_3084648
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06239
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
FC protocol for CL594 LCK antibody CL594-60162Download protocol
Standard Protocols
Click here to view our Standard Protocols