CoraLite®594-conjugated LCK Monoclonal antibody
LCK Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, pig, rat
Applications
FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2D10C2
Cat no : CL594-60162
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | A431 cells |
Positive FC detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-60162 targets LCK in FC (Intra) applications and shows reactivity with human, pig, rat samples.
Tested Reactivity | human, pig, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | LCK fusion protein Ag7694 相同性解析による交差性が予測される生物種 |
Full Name | lymphocyte-specific protein tyrosine kinase |
Calculated molecular weight | 539 aa, 56 kDa |
Observed molecular weight | 50-60 kDa |
GenBank accession number | BC013200 |
Gene symbol | LCK |
Gene ID (NCBI) | 3932 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
---|---|
FC protocol for CL594 LCK antibody CL594-60162 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |