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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LENG9 Polyclonal antibody

LENG9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16295-1-AP

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using LENG9 Polyclonal antibody (16295-1-AP)

    WB analysis of HeLa using 16295-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16295-1-AP (LENG9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using LENG9 Polyclonal antibody (16295-1-AP)

    WB analysis of Y79 using 16295-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 16295-1-AP (LENG9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human kidney using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human kidney using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human lung using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human lung using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human placenta using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human placenta using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human testis using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human testis using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human skin using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human skin using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human brain using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human brain using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human liver using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human liver using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human spleen using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human spleen using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human heart using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human heart using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using LENG9 Polyclonal antibody (16295-1-AP)

    IF Staining of MCF-7 using 16295-1-AP

    Immunofluorescent analysis of MCF-7 cells, using LENG9 antibody 16295-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LENG9 Polyclonal antibody (16295-1-AP)

    IF Staining of HeLa using 16295-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LENG9 antibody (16295-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, Y79 cells
Positive IHC detected inhuman kidney tissue, human brain tissue, human heart tissue, human liver tissue, human lung tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16295-1-AP targets LENG9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LENG9 fusion protein Ag9481 相同性解析による交差性が予測される生物種
Full Name leukocyte receptor cluster (LRC) member 9
Calculated molecular weight 501 aa, 53 kDa
Observed molecular weight 54-60 kDa
GenBank accession numberBC015921
Gene symbol LENG9
Gene ID (NCBI) 94059
RRIDAB_2296934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LENG9 is rarely reported in the literature. GO function analysis revealed that the molecular function of LENG9 is metal ion binding (GO:0046872), and the BioGRID database showed that LENG9 has 6 interactors, including C9ORF41, B4GALT2, CDC5L, D2HGDH, FOXS1 and OGT. Studies have shown that LENG9 may play an oncogenic role in tumor pathogenesis by promoting tumor cell proliferation, migration, and invasion. (PMID: 35538075)

Protocols

Product Specific Protocols
WB protocol for LENG9 antibody 16295-1-APDownload protocol
IHC protocol for LENG9 antibody 16295-1-APDownload protocol
IF protocol for LENG9 antibody 16295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Long-read sequencing unveils high-resolution HPV integration and its oncogenic progression in cervical cancer.

Authors - Liyuan Zhou
View Article