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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated LENG9 Polyclonal antibody

LENG9 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16295

Synonyms

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated LENG9 Polyclonal ant (CL488-16295)

    IF Staining of HeLa using CL488-16295

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 LENG9 antibody (CL488-16295) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated LENG9 Polyclonal ant (CL488-16295)

    FC experiment of HeLa using CL488-16295

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated LENG9 Polyclonal antibody (CL488-16295)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-16295 targets LENG9 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LENG9 fusion protein Ag9481 相同性解析による交差性が予測される生物種
Full Name leukocyte receptor cluster (LRC) member 9
Calculated molecular weight 501 aa, 53 kDa
Observed molecular weight 54-60 kDa
GenBank accession numberBC015921
Gene symbol LENG9
Gene ID (NCBI) 94059
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LENG9 is rarely reported in the literature. GO function analysis revealed that the molecular function of LENG9 is metal ion binding (GO:0046872), and the BioGRID database showed that LENG9 has 6 interactors, including C9ORF41, B4GALT2, CDC5L, D2HGDH, FOXS1 and OGT. Studies have shown that LENG9 may play an oncogenic role in tumor pathogenesis by promoting tumor cell proliferation, migration, and invasion. (PMID: 35538075)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 LENG9 antibody CL488-16295Download protocol
FC protocol for CL Plus 488 LENG9 antibody CL488-16295Download protocol
Standard Protocols
Click here to view our Standard Protocols