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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LENG9 Polyclonal antibody (16295-1-AP)

    WB analysis of HeLa using 16295-1-AP (same clone as 16295-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16295-1-AP (LENG9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using LENG9 Polyclonal antibody (16295-1-AP)

    WB analysis of Y79 using 16295-1-AP (same clone as 16295-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 16295-1-AP (LENG9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human kidney using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human kidney using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human placenta using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human placenta using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human testis using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human testis using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human skin using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human skin using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human brain using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human brain using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human liver using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human liver using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human spleen using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human spleen using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human lung using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human lung using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human heart using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using LENG9 Polyclonal antibody (16295-1-AP)

    IHC staining of human heart using 16295-1-AP (same clone as 16295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LENG9 Polyclonal antibody (16295-1-AP)

    IF Staining of HeLa using 16295-1-AP (same clone as 16295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LENG9 antibody (16295-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using LENG9 Polyclonal antibody (16295-1-AP)

    IF Staining of MCF-7 using 16295-1-AP (same clone as 16295-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using LENG9 antibody 16295-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16295-1-PBS in a different storage buffer formulation.

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LENG9 Polyclonal antibody, PBS Only

LENG9 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16295-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16295-1-PBS targets LENG9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LENG9 fusion protein Ag9481 相同性解析による交差性が予測される生物種
Full Name leukocyte receptor cluster (LRC) member 9
Calculated molecular weight 501 aa, 53 kDa
Observed molecular weight 54-60 kDa
GenBank accession numberBC015921
Gene Symbol LENG9
Gene ID (NCBI) 94059
RRIDAB_2296934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96B70
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

LENG9 is rarely reported in the literature. GO function analysis revealed that the molecular function of LENG9 is metal ion binding (GO:0046872), and the BioGRID database showed that LENG9 has 6 interactors, including C9ORF41, B4GALT2, CDC5L, D2HGDH, FOXS1 and OGT. Studies have shown that LENG9 may play an oncogenic role in tumor pathogenesis by promoting tumor cell proliferation, migration, and invasion. (PMID: 35538075)