Galectin-4 Monoclonal antibody
Galectin-4 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
3C2G3
Cat no : 66686-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HT-29 cells, human ileum tissue |
Positive IHC detected in | human colon tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human colon cancer tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:5000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66686-1-Ig targets Galectin-4 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Galectin-4 fusion protein Ag4049 相同性解析による交差性が予測される生物種 |
Full Name | lectin, galactoside-binding, soluble, 4 |
Calculated molecular weight | 323 aa, 36 kDa |
Observed molecular weight | 36 kDa |
GenBank accession number | BC034750 |
Gene symbol | Galectin-4 |
Gene ID (NCBI) | 3960 |
RRID | AB_2882040 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.
Protocols
Product Specific Protocols | |
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WB protocol for Galectin-4 antibody 66686-1-Ig | Download protocol |
IHC protocol for Galectin-4 antibody 66686-1-Ig | Download protocol |
IF protocol for Galectin-4 antibody 66686-1-Ig | Download protocol |
FC protocol for Galectin-4 antibody 66686-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |