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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Galectin-4 Monoclonal antibody

Galectin-4 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C2G3

Cat no : CL594-66686

Synonyms

Antigen NY CO 27, Gal 4, GAL4, Galectin 4, Galectin-4, L 36 lactose binding protein, L36LBP, Lactose binding lectin 4, LGALS4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite®594-conjugated Galectin-4 Monoclonal anti (CL594-66686)

    IF Staining of human colon cancer using CL594-66686

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Galectin-4 antibody (CL594-66686, Clone: 3C2G3 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite®594-conjugated Galectin-4 Monoclonal anti (CL594-66686)

    IF Staining of human colon cancer using CL594-66686

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Galectin-4 antibody (CL594-66686, Clone: 3C2G3 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inhuman colon cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66686 targets Galectin-4 in IF-P applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Galectin-4 fusion protein Ag4049 相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 4
Calculated molecular weight 323 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC034750
Gene symbol Galectin-4
Gene ID (NCBI) 3960
RRIDAB_2920023
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.

Protocols

Product Specific Protocols
IF protocol for CL594 Galectin-4 antibody CL594-66686Download protocol
Standard Protocols
Click here to view our Standard Protocols