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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using CoraLite®594-conjugated LGMN Monoclonal antibody (CL594-67017)

    IF Staining of human kidney using CL594-67017

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 LGMN antibody (CL594-67017, Clone: 1F2B12 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using CoraLite®594-conjugated LGMN Monoclonal antibody (CL594-67017)

    IF Staining of human kidney using CL594-67017

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 LGMN antibody (CL594-67017, Clone: 1F2B12 ) at dilution of 1:200.

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  • KD/KO Validated

CoraLite®594-conjugated LGMN Monoclonal antibody

LGMN Monoclonal Antibody for IF-P
Cat No. CL594-67017
Clone No.1F2B12

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P

Synonyms

AEP, Asparaginyl endopeptidase, legumain, LGMN, LGMN1, Protease, cysteine 1, PRSC1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman kidney tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67017 targets LGMN in IF-P applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LGMN fusion protein Ag7218 相同性解析による交差性が予測される生物種
Full Name legumain
Calculated molecular weight 49 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC003061
Gene Symbol LGMN
Gene ID (NCBI) 5641
RRIDAB_2920072
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99538
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471).

Protocols

Product Specific Protocols
IF protocol for CL594 LGMN antibody CL594-67017Download protocol
Standard Protocols
Click here to view our Standard Protocols