CoraLite® Plus 488-conjugated LIAS Monoclonal antibody
LIAS Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3B5G7
Cat no : CL488-67298
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67298 targets LIAS in FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | LIAS fusion protein Ag27286 相同性解析による交差性が予測される生物種 |
| Full Name | lipoic acid synthetase |
| Calculated molecular weight | 372 aa, 42 kDa |
| Observed molecular weight | 34-42 kDa |
| GenBank accession number | BC023635 |
| Gene symbol | LIAS |
| Gene ID (NCBI) | 11019 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LIAS(lipoyl synthase, mitochondrial) is also named as LAS and belongs to the radical SAM superfamily and lipoyl synthase family. It produces alpha-lipoic acid, an antioxidant and an essential cofactor in alpha-ketoacid dehydrogenase complexes, which participate in glucose oxidation and ATP generation(PMID:22021711). The deduced 373-amino acid protein has a calculated molecular mass of about 42 kD. The N-terminal 26 amino acids encode a potential mitochondrial targeting presequence that, upon removal, would result in a deduced mature protein of 347 amino acids with a molecular mass of about 39 kD(PMID:11389890). Defects in LIAS are a cause of pyruvate dehydrogenase lipoic acid synthetase deficiency (PDHLD).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 LIAS antibody CL488-67298 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
