Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, Jurkat cells, MOLT-4 cells, K-562 cells, HSC-T6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
67840-1-Ig targets LIG1 in WB, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | LIG1 fusion protein Ag12489 相同性解析による交差性が予測される生物種 |
| Full Name | ligase I, DNA, ATP-dependent |
| Calculated molecular weight | 919 aa, 102 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | BC108318 |
| Gene Symbol | LIG1 |
| Gene ID (NCBI) | 3978 |
| RRID | AB_2918602 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P18858 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DNA ligase I (LIG1) joins DNA strand breaks during DNA replication and repair transactions and contributes to genome integrity. 67840-1-Ig is raised agains the C-terminal 670-919 aa residues of the human DNA ligase 1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LIG1 antibody 67840-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|