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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LIG1 Monoclonal antibody (67840-1-Ig)

    WB analysis using 67840-1-Ig (same clone as 67840-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67840-1-Ig (LIG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67840-1-PBS in a different storage buffer formulation.

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LIG1 Monoclonal antibody, PBS Only

LIG1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67840-1-PBS
Clone No.3D9G8

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

DNA ligase 1, DNA ligase I, LIG1, ligase I, DNA, ATP dependent

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67840-1-PBS targets LIG1 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen LIG1 fusion protein Ag12489 相同性解析による交差性が予測される生物種
Full Name ligase I, DNA, ATP-dependent
Calculated molecular weight 919 aa, 102 kDa
Observed molecular weight130 kDa
GenBank accession numberBC108318
Gene Symbol LIG1
Gene ID (NCBI) 3978
RRIDAB_2918602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18858
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DNA ligase I (LIG1) joins DNA strand breaks during DNA replication and repair transactions and contributes to genome integrity. 67840-1-Ig is raised agains the C-terminal 670-919 aa residues of the human DNA ligase 1.