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DNA ligase III/LIG3 Monoclonal antibody

DNA ligase III/LIG3 Monoclonal Antibody for WB, ELISA
Cat No. 68810-1-Ig
Clone No.1B10B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

LIG3, LIG2, EC:6.5.1.1, DNA ligase III, DNA ligase 3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68810-1-Ig targets DNA ligase III/LIG3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DNA ligase III/LIG3 fusion protein Ag31484 相同性解析による交差性が予測される生物種
Full Name ligase III, DNA, ATP-dependent
Calculated molecular weight 100-110 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC068005
Gene Symbol LIG3
Gene ID (NCBI) 3980
RRIDAB_3670431
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49916
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNA ligase III (DNA ligase 3) is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.

Protocols

Product Specific Protocols
WB protocol for DNA ligase III/LIG3 antibody 68810-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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